Comparison of carminic acid extraction by UAE and MAE techniques under the same in vitro conditions
Keywords:
carminic acid, cochineal, ultrasound, microwave, optimizationAbstract
Carminic acid is a highly valuable natural dye extracted from the cochineal insect (Dactylopius coccus), widely used in food, cosmetics, and textile industries. The limitations of conventional extraction methods such as high energy consumption and intensive use of solvents have driven interest in more sustainable technologies. This article presents an exploratory and descriptive literature review comparing two green extraction techniques: ultrasound-assisted extraction (UAE) and microwave assisted extraction (MAE) under controlled laboratory conditions. A total of 30 scientific publications from recognized academic databases were analyzed. The results indicate that UAE achieved a carminic acid yield of 26.3%, compared to 18% for MAE. Additionally, extracts obtained through microwave treatment exhibited greater color intensity in textile applications, provided that irradiation was not excessive. Further studies revealed that a microwave power of 400 W was optimal for extraction, as higher power levels compromised cellular structure and reduced efficiency. These findings confirm that optimizing parameters such as power, temperature, exposure time, and solid-to-liquid ratio is critical for improving extraction efficiency in future industrial applications. Both techniques demonstrated significant advantages over traditional methods, positioning themselves as viable alternatives for enhancing efficiency and sustainability in the production of natural dyes.
References
Adeel, S., Hussaan, M., Rehman, F. ur, Habib, N., Salman, M., Naz, S., Amin, N., & Akhtar, N. (2019). Microwave-assisted sustainable dyeing of wool fabric using cochineal-based carminic acid as natural colorant. Journal of Natural Fibers, 16(7), 1026–1034. https://doi.org/10.1080/15440478.2018.1448317
Ahmed, H. M., Ahmed, K. A., Mashaly, H. M., & El-Halwagy, A. A. (2017). Treatment of Cotton Fabric with Dielectric Barrier Discharge (DBD) Plasma and Printing with Cochineal Natural Dye. Indian Journal of Science and Technology, 10(10), 1–10. https://doi.org/10.17485/ijst/2017/v10i10/100973
Amin, N., & Rehman, F.-. (2019). Sustainable application of cochineal-based anthraquinone dye for the coloration of bio-mordanted silk fabric. 6851–6860. https://doi.org/https://doi.org/10.1007/s11356-019-06868-3
Benbelkhir, F. Z., & Medjekal, S. (2022). Microalgal carotenoids: A promising alternative to synthetic dyes. Algal Research, 66(July), 102823. https://doi.org/10.1016/j.algal.2022.102823
Borges, M. E., Tejera, R. L., Díaz, L., Esparza, P., & Ibáñez, E. (2012). Natural dyes extraction from cochineal (Dactylopius coccus). New extraction methods. Food Chemistry, 132(4), 1855–1860. https://doi.org/10.1016/j.foodchem.2011.12.018
Carrasco Vera, C. J., Santana Aragone, D. X., González Chica, M. Y., Villamarin Barreiro, J. J., Vásquez Moran, V. F., & Coello Mieles, A. J. (2024). Plan de mejora para el control de cochinillas en cultivo de banano en la zona Los Ríos, Ecuador. Conocimiento Global, 9(1), 1–17. https://doi.org/10.70165/cglobal.v9i1.324
Deveoglu, O. (2020). A review on cochineal (Dactylopius Coccus Costa) dye. Research Journal of Recent Sciences, 9(3), 37–43. www.isca.me
Domínguez-Castillo, C., Jiménez-Hidalgo, M., López-Gámez, J., Rodríguez-Hortal, A., Alzaga-García, M., Gallardo-Abárzuza, M., Higueras-Milena, J. M., Gómez-Morón, A., García-Viñas, E., Bernáldez-Sánchez, E., & Martínez-Haya, B. (2023). High-resolution mass spectrometry identification of dye compounds and their degradation products in American cochineal from a historic shipping cargo. Dyes and Pigments, 216(April). https://doi.org/10.1016/j.dyepig.2023.111313
El-Hawary, N. (2019). High-Performance Natural Dyes for Cellulosic Fibers Review - part 1. Journal of Textiles, Coloration and Polymer Science, 0(0), 0–0. https://doi.org/10.21608/jtcps.2019.8154.1018
Ferreyra-Suarez, D., Paredes-Vargas, L., Jafari, S. M., García-Depraect, O., & Castro-Muñoz, R. (2024). Extraction pathways and purification strategies towards carminic acid as natural-based food colorant: A comprehensive review. Advances in Colloid and Interface Science, 323(November 2023), 103052. https://doi.org/10.1016/j.cis.2023.103052
Flores-Hernández, A., Murillo-Amador, B., Rueda-Puente, E. O., Salazar-Torres, J. C., García-Hernández, J. L., & Troyo-Diéguez, E. (2006). Reproducción de cochinilla silvestre Dactylopius opuntiae (Homóptera: Dactylopiidae). Revista Mexicana de Biodiversidad, 77(1), 97–102. https://doi.org/10.22201/ib.20078706e.2006.001.321
García-Lapuente, J., Olvera-Quintanar, E., Tapia-Mendoza, E., & Cruz-Moreno, G. A. (2025). Optimization of red hue in wool dyeing using Mexican cochineal through sequential experimental designs. Journal of the Textile Institute, 0(0), 1–10. https://doi.org/10.1080/00405000.2025.2457581
Garzón, G. A. (2008). Las Antocianinas Como Colorantes Naturales y Compuestos Bioactivos: Revisión. 13(3), 27–36.
Guo, Y., Zheng, H., Zhang, H., Ma, L., Han, J., & Li, K. (2012). Optimization of combined microwave-ultrasonic wave extraction of cochineal dye by response surface methodology. Applied Mechanics and Materials, 161, 82–87. https://doi.org/10.4028/www.scientific.net/AMM.161.82
Isabel Landim Neves, M., Keven Silva, E., & Angela A. Meireles, M. (2019). Trends and Challenges in the Industrialization of Natural Colorants. Food and Public Health, 9(2), 33–44. https://doi.org/10.5923/j.fph.20190902.01
Linares, G., & Rojas, M. L. (2022). Ultrasound-Assisted Extraction of Natural Pigments From Food Processing By-Products: A Review. Frontiers in Nutrition, 9(May), 1–17. https://doi.org/10.3389/fnut.2022.891462
Manzoor, M., Singh, J., Gani, A., & Noor, N. (2021). Valorization of natural colors as health-promoting bioactive compounds: Phytochemical profile, extraction techniques, and pharmacological perspectives. Food Chemistry, 362, 130141. https://doi.org/10.1016/j.foodchem.2021.130141
Mostacero-León, J., De La Cruz-Castillo, A., Taramona-Ruíz, L., Alva Calderón, R., Seijas-Bernabé, P., & Mendoza Rodríguez, R. (2020). Efecto citotóxico del colorante alimentario rojo 40 en nauplios de Artemia salina Leach. Revista de Investigaciones de La Universidad Le Cordon Bleu, 7(2), 60–66. https://doi.org/10.36955/riulcb.2020v7n2.006
Nonglait, D. L., & Gokhale, J. S. (2024). Review Insights on the Demand for Natural Pigments and Their Recovery by Emerging Microwave-Assisted Extraction (MAE). Food and Bioprocess Technology, 17(7), 1681–1705. https://doi.org/10.1007/s11947-023-03192-0
Ochoa Manzo, G. M., Martínez Flores, H. E., Rodiles López, J. O., & Portillo, L. (2025). Carmine red from cochineal (Dactylopius coccus), a natural dye: a review. Ciencia Nicolaita, 93, 26–34. https://doi.org/10.35830/cn.vi93.827
Olvera-Quintanar, E., García-Lapuente, J., Cruz-Moreno G., A., & Tapia-Mendoza, E. (2025). Optimisation of purple dyeing with Mexican cochineal in cotton with multivariate analysis and the response surface method. Coloration Technology, August 2024, 1–14. https://doi.org/10.1111/cote.12822
Rehman, F. U., Adeel, S., Pervaiz, M., Haji, A., Haddar, W., Hussaan, M., Amin, N., & Guesmi, A. (2021). Microwave Induced Sustainable Isolation of Laccaic Acid from Lac Insect for Nylon Dyeing. Iranian Journal of Chemistry and Chemical Engineering, 40(6), 1849–1859. https://doi.org/10.30492/ijcce.2020.119496.3907
Reyes-Pérez, R., Pérez-Hernández, J., Rosas-Morales, M., Plascencia-Espinosa, M. Á., Lazo-Zamalloa, O., López-Gayou, V., López, P. A., Ríos-Cortés, G., & Ríos-Cortés, A. M. (2024). Cochineal (Dactylopius coccus Costa) Pigment Extraction Assisted by Ultrasound and Microwave Techniques. Molecules, 29(23). https://doi.org/10.3390/molecules29235568
Sigurdson, G. T., Tang, P., & Giusti, M. M. (2017). Natural Colorants: Food Colorants from Natural Sources. Annual Review of Food Science and Technology, 8, 261–280. https://doi.org/10.1146/annurev-food-030216-025923
Vakte, S. R., Sonawane, C. P., Saraf, K. V, Kashmire, S. M., & Nehete, J. Y. (2024). Cochineal insects ( Dactylopius coccus ) in cosmetics : An overview of taxonomy , composition , extraction methods , applications , and regulatory perspectives extraction methods , applications , and regulatory perspectives. International Journal of Entomology Research, 9(October).
Downloads
Published
Issue
Section
License
Copyright (c) 2025 Diana Alexandra Castillo Villota, Joel Adrian Izurieta Reyes, Juan Andres Saldaña Bueno, Wilson Patricio León Cueva (Autor/a)

This work is licensed under a Creative Commons Attribution 4.0 International License.
Los artículos publicados en la revista se distribuyen bajo la licencia Creative Commons Atribución 4.0 Internacional (CC BY 4.0). Esta licencia permite a terceros descargar, copiar, distribuir, adaptar y reutilizar una obra, incluso con fines comerciales, siempre que se otorgue el crédito adecuado al autor original.